|
KMID : 0370220070510050291
|
|
Yakhak Hoeji
2007 Volume.51 No. 5 p.291 ~ p.295
|
|
|
Role of Akt in Insulin-Mediated Increase in Expression of Microsomal Epoxide Hydrolase
|
|
Kim Sang-Kyum
Kang Keon-Wook
Kim Bong-Hee
Oh Jung-Min
Yun Kang-Uk
Kim Chung-Hyeon
|
|
|
Abstract
|
|
|
|
The present study examines the effect of dominant-negative Akt on the insulin-mediated microsomal epoxide hydrolase (mEH) induction in rat hepatocytes. We also assessed the role of insulin in the expression of soluble epoxide hydrrolase (sEH). Insulin increased mEH levels and the enzyme activities, whereas sEH protein expression was unaffected by insulin. The specific PI3K inhibitors or p70 S6 kinase inhibitor ameliorated the insulin-mediated increase in mEH protein levels. Infection with adenovirus expressing dominant-negative and kinase-dead mutant of Akt1 effectively inhibited the insulin-mediated increase in mEH expression and mEH activity. These results suggest that mEH and sEH are differentially regulated by insulin and PI3K/Akt/p70S6K are active in the insulin-mediated regulation of mEH expression.
|
|
|
KEYWORD
|
|
|
microsomal epoxide hydrolase, soluble epoxide hydrolase, insulin, cellular signaling pathway, akt
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|